BRM270 Inhibits the Proliferation of CD44 Positive Pancreatic Ductal Adenocarcinoma Cells via Downregulation of Sonic Hedgehog Signaling

Figure 1

Isolation of CD44⁺ PDAC cells. (a) FACS analysis of CD44 surface marker in positive and negative CD44 BxPC-3 and PANC-1 after MACS separation. (b) Western blotting of lysates after MACS separation. (b) Immunocytochemistry staining of CD44, scale bar 20 μm. (d) Comparison of CD44⁺ and induced tumor sizes. (e) Tumor weights of CD44⁺ and PDAC cells. Significant differences of values are compared to values of negative and positive CD44 cells and marked as follows: P< 0.05; P< 0.01; P< 0.001; P< 0.0001.