BRM270 Inhibits the Proliferation of CD44 Positive Pancreatic Ductal Adenocarcinoma Cells via Downregulation of Sonic Hedgehog Signaling

Figure 2

BRM270 suppresses the malignance of CD44⁺ PDAC cells. (a) Dose-dependent inhibitory effects of BRM270 to CD44⁺ PDAC cells. (b) The apoptosis of CD44⁺ PDAC cells exposed by BRM270, detected by FACS Annexin V. (c) Western blotting of apoptotic proteins in lysates with and without 50 μg/mL BRM270 treatment. (d) Clonogenic assay of CD44⁺ PDAC cells with and without 50 μg/mL BRM270 treatment. (e) Migration and invasion assay of CD44⁺ PDAC cells with and without 50 μg/mL BRM270 treatment, scale bar 100 μm. (f) Wound healing assay of CD44⁺ PDAC cells with and without 50 μg/mL BRM270 treatment. Significant differences of values are compared to values of positive CD44 cells with and without BRM270 treatment and marked as follows: P< 0.0001.