Simultaneous Determination of Three Bioactive Constituents from <i>Bletilla striata</i> by UPLC-MS/MS and Application of the Technique to Pharmacokinetic Analyses

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr class="thead"><td class="align_left">Component</td><td class="align_center">Transition (<i>m/z</i>)</td><td class="align_center">Cone voltage (V)</td><td class="align_center">Collision energy (eV)</td></tr><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr><td class="align_left"><i>α</i>-Isobutylmalic acid</td><td class="align_center">189 ⟶ 129</td><td class="align_center">30</td><td class="align_center">15</td></tr><tr><td class="align_left">Gymnoside I</td><td class="align_center">457.2 ⟶ 285.1</td><td class="align_center">35</td><td class="align_center">15</td></tr><tr><td class="align_left">Militarine</td><td class="align_center">725.3 ⟶ 457.2</td><td class="align_center">40</td><td class="align_center">20</td></tr><tr><td class="align_left">Puerarin (IS)</td><td class="align_center">417.0 ⟶ 267.0</td><td class="align_center">40</td><td class="align_center">30</td></tr><tr class="table-tr"><td colspan="4"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Precursor/product ion pairs and parameters for MRM of the analytes and the internal standard (IS) puerarin.</div>

Evidence-Based Complementary and Alternative Medicine

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Table 1

Table 1: Simultaneous Determination of Three Bioactive Constituents from <i>Bletilla striata</i> by UPLC-MS/MS and Application of the Technique to Pharmacokinetic Analyses 

Table 1 | Simultaneous Determination of Three Bioactive Constituents from Bletilla striata by UPLC-MS/MS and Application of the Technique to Pharmacokinetic Analyses 