Simultaneous Determination of Three Bioactive Constituents from Bletilla striata by UPLC-MS/MS and Application of the Technique to Pharmacokinetic Analyses

Table 3

Summary of precision and accuracy of quality control samples added to rat plasma (n = 5).

Analytes	Spiked concentration (μg/mL)	Intraday			Interday
		Calculated concentration (μg/mL)	Precision (RSD%)	Accuracy (%)	Calculated concentration (ng/mL)	Precision (RSD,%)	Accuracy (%)
α-Isobutylmalic acid	0.029	0.029 ± 0.001	6.5	101.3	0.028 ± 0.001	5.34	96.6
	4.09	3.84 ± 0.04	10.4	93.9	3.83 ± 0.05	1.3	99.7
	36.78	36.29 ± 1.45	4.0	98.7	36.11 ± 1.21	3.4	98.2
Gymnoside I	0.02	0.02 ± 0.001	4.6	100.9	0.02 ± 0.001	5.5	100.4
	2.75	2.62 ± 0.09	3.3	95.4	2.60 ± 0.09	3.5	94.5
	13.75	13.19 ± 0.02	1.5	95.9	13.45 ± 0.37	2.8	97.8
Militarine	0.015	0.014 ± 0.002	11.4	93.3	0.014 ± 0.001	8.4	93.3
	2.64	2.57 ± 0.05	1.9	97.3	2.60 ± 0.06	2.3	98.5
	23.78	23.03 ± 1.34	5.8	96.8	23.29 ± 1.13	4.9	97.9