Simultaneous Determination of Three Bioactive Constituents from Bletilla striata by UPLC-MS/MS and Application of the Technique to Pharmacokinetic Analyses

Table 4

Matrix effects and extraction recoveries of the three compounds (n = 5).

Analytes	Spiked concentration (ng/mL)	Extraction recovery		Matrix effect
		Mean ± SD (%)	RSD (%)	Mean ± SD (%)	RSD (%)
α-Isobutylmalic acid	0.029	99.68 ± 4.50	4.5	93.21 ± 3.54	3.8
	4.09	95.77 ± 1.12	1.2	96.45 ± 0.78	0.8
	36.78	100.98 ± 2.14	2.1	100.09 ± 2.45	2.4
Gymnoside I	0.22	93.99 ± 6.47	6.9	98.73 ± 7.59	7.7
	2.75	92.65 ± 8.09	8.7	101.74 ± 3.34	3.3
	13.75	94.71 ± 4.03	4.3	99.08 ± 0.67	0.7
Militarine	0.015	100.16 ± 8.81	8.8	94.81 ± 1.56	1.6
	2.64	94.78 ± 2.17	2.3	95.63 ± 2.53	2.6
	23.78	99.66 ± 5.36	5.4	99.91 ± 6.36	6.4