Simultaneous Determination of Three Bioactive Constituents from Bletilla striata by UPLC-MS/MS and Application of the Technique to Pharmacokinetic Analyses

Table 5

Summary of stability of quality control samples added to rat plasma (n = 5).

Analytes concentration (μg/mL)	Spiked concentration (μg/mL)	12 h, 4°C			3 freeze-thaw cycles			6 h, room temperature
		Calculated concentration (μg/mL)	Precision (RSD, %)	Accuracy (%)	Calculated concentration (μg/mL)	Precision (RSD, %)	Accuracy (%)	Calculated concentration (μg/mL)	Precision (RSD, %)	Accuracy (%)
α-Isobutylmalic acid	0.029	0.028 ± 0.002	7.1	96.6	0.027 ± 0.001	3.7	93.1	0.03 ± 0.001	3.3	103.4
	4.09	3.92 ± 0.11	2.8	95.8	3.83 ± 0.08	10.4	93.6	3.77 ± 0.09	2.4	92.2
	36.78	34.71 ± 1.95	6.1	94.4	34.17 ± 1.28	3.7	92.9	34.95 ± 1.89	5.4	95.0
Gymnoside I	0.022	0.021 ± 0.001	4.8	95.4	0.022 ± 0.001	4.8	100.3	0.022 ± 0.001	4.5	100.2
	2.75	2.68 ± 0.15	6.0	97.5	2.74 ± 0.10	3.6	99.6	2.77 ± 0.08	3.6	99.6
	13.75	13.22 ± 0.49	3.7	96.1	13.92 ± 0.57	5.2	101.2	13.69 ± 0.39	5.2	101.2
Militarine	0.015	0.015 ± 0.001	6.7	100.1	0.015 ± 0.001	6.7	100.7	0.016 ± 0.001	6.2	106.7
	2.64	2.46 ± 0.28	11.4	93.2	2.62 ± 0.09	3.4	99.2	2.62 ± 0.09	3.4	99.2
	23.78	21.95 ± 1.1	5.0	92.3	21.8 ± 0.94	4.3	91.7	21.3 ± 0.94	4.4	89.6