Simultaneous Determination of Three Bioactive Constituents from Bletilla striata by UPLC-MS/MS and Application of the Technique to Pharmacokinetic Analyses

Table 6

Pharmacokinetic parameters of the three analytes after oral and intravenous administration of B. striata extract to rats (n = 6).

Parameters	Unit	Intravenous			Oral
		α-Isobutylmalic acid	Gymnoside I	Militarine	α-Isobutylmalic acid	Gymnoside I	Militarine
Tmax	h	—	—	—	6.44 ± 2.33	1.44 ± 2.29	0.16 ± 0.11
Cmax	μg/mL	—	—	—	23.18 ± 5.42	3.06 ± 4.82	3.30 ± 3.60
AUC0–t	hμg/mL	38.7 ± 4.72	15.47 ± 1.63	36.71 ± 5.66	458.12 ± 150.74	20.56 ± 12.05	3.10 ± 1.16
AUC0–∞	hμg/mL	39.62 ± 4.26	17.52 ± 2.66	37.22 ± 5.99	498.36 ± 173.09	21.81 ± 12.04	4.26 ± 1.93
MRT0–t	h	0.45 ± 0.08	0.68 ± 0.07	0.36 ± 0.06	15.59 ± 3.63	12.42 ± 3.73	5.62 ± 2.45
MRT0–∞	h	0.51 ± 0.1	0.93 ± 0.18	0.39 ± 0.07	19.69 ± 6.25	15.98 ± 5.34	9.31 ± 2.57
Bioavailability	%	—	—	—	87.4%	9.8%	0.6%