Anticancer Effect of the Ethyl Acetate Fraction from Orostachys japonicus on MDA-MB-231 Human Breast Cancer Cells through Extensive Induction of Apoptosis, Cell Cycle Arrest, and Antimetastasis

Figure 5

Effect of OJEF on the protein levels of (a) bcl-2, pro-caspase-3, -8, -9, and -12, and cleaved caspase-3 and -9, (b) CDK2, CDK4, cyclin D1, and cyclin B1, (c) claudin-1, zo-1, E-cadherin, β-catenin, integrin β1, and MMP-9, and (d) MAPK family in MDA-MB-231 human breast cancer cells. The cells were treated with OJEF (0, 0.1% DMSO, 10, 20, 40, or 60 µg/mL) for 12 h. Expression of the indicated proteins was examined by western blotting. The density of bands was quantitated. GAPDH was used as an internal control. Band intensities were measured by densitometry in three separate experiments with similar results. The values are indicated as the means ± S.D. (n = 3). Values of , , and were considered statistically meaningful.

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