Comparative Effect of Aqueous and Methanolic Bupleuri Radix Extracts on Hepatic Uptake of High-Density Lipoprotein and Identification of the Potential Target in HFD-Fed Mice

Figure 4

Screening the bioactive ingredients in Bupleuri Radix extract by membrane immobilized chromatography. (a) Superposed HPLC-DAD chromatograms of SSd standard, SSa standard, Bupleuri Radix aqueous extract, first PBS elution, ninth PBS elution, and desorption solution in sequence from the top to bottom detected at wavelength 204 nm. No compounds were found in the desorption solution. (b) Superposed HPLC-DAD chromatograms of SSb₂ standard, SSb₁ standard, Bupleuri Radix aqueous extract, first PBS elution, second PBS elution, ninth PBS elution, and desorption solution in sequence from the top to bottom detected at wavelength 254 nm. Compound I and II were found in the desorption solution of the HepG2 cell membrane, which showed the same retention time with standards SSb₂ and SSb_1, respectively. (c, d) Secondary ion current chromatogram of LC-Q-Tof/MS/MS verified compound I was SSb₂ and compound II was SSb₁.

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