Huangqi-Danshen Decoction Ameliorates Adenine-Induced Chronic Kidney Disease by Modulating Mitochondrial Dynamics

Figure 4

HDD decreased mitochondrial fission and increased mitochondrial fusion in CKD rats. (a) Representative Western blot images of Drp-1, Mid 51, and Mid 49. (b) Densitometric analysis of Drp-1, Mid 51, and Mid 49 protein expression, normalized to GAPDH content. (c) Representative immunofluorescence images indicating the colocalization of Drp-1 and TOM 20. Green corresponds to Drp-1, red corresponds to TOM 20, and blue corresponds to nuclear staining. All images are shown at identical magnification, ×200, scale bar=100 μm. (d) Representative Western blot images of Mfn-2 and OPA-1 spectrum. (e) Densitometric analysis of Mfn-2, L-OPA-1, and S-OPA-1 protein expression, normalized to GAPDH content. Data are presented as the means ± SEM, n=6 rats per group (, compared with the control group; ^#P < 0.05, ^##P < 0.01 compared with the CKD group).

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