Cytotoxic Property of Grias neuberthii Extract on Human Colon Cancer Cells: A Crucial Role of Autophagy

Figure 2

GNSbM extract induces autophagy on RKO line cell. Cells were exposed to GNSbM or UV radiation or starvation in PBS. Total protein was separated in a SDS-PAGE followed by Western blot analysis with indicated antibodies against p53, apoptotic and autophagy biomarkers, and tubulin as a loading control. (a) Quantification of the level of p53 expression and the apoptotic and autophagy biomarkers. Data represented the mean ± SEM (n = 6) of three independent experiments. The tests for significance were limited to ANOVA-Dunnet posttest: , , vs. control. (b) Western blot pictures demonstrating the effect observed in RKO cell line. Although p53 showed to be overexpressed in a dose-dependent manner, no apoptotic activity was detected, and autophagy activity was monitored after treatment.

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