Cytotoxic Property of Grias neuberthii Extract on Human Colon Cancer Cells: A Crucial Role of Autophagy

Figure 3

GNSbM extract induces autophagy on SW613-B3 cell line. Cells were exposed to GNSbM or UV radiation or starvation in PBS. Total protein was separated in a SDS-PAGE followed by Western blot analysis with indicated antibodies against p53, apoptotic and autophagy biomarkers, and tubulin as a loading control. (a) Quantification of the level of p53 expression and the apoptotic and autophagy biomarkers. Data represented the mean ± SEM (n = 6) of three independent experiments. The tests for significance were limited to the ANOVA-Dunnet posttest: , , vs. control. (b) Western blot pictures demonstrating the effect observed in Sw613-B3 cell line. No p53 expression was observed as expected although autophagy activity was detected after treatment.

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