Discovery of Quality Markers in Hugan Qingzhi Formula by Integrating a Lipid-Lowering Bioassay with UHPLC-QQQ-MS/MS

Table 1

The calibration curves, LOD, LOQ, precision, repeatability, stability, and recovery of 12 compounds.

Analyte	Linearity			LOD (ng/mL)	LOQ (ng/mL)	Precision RSD (%) (n = 6)	Repeatability RSD (%) (n = 6)	Stability RSD (%) (n = 6)	Recovery RSD (%) (n = 6)
	Standard curves	R^2	Range (µg/mL)						Original (μg)	Spiked (µg)	Found (gµ)	Recovery (%)	RSD (%)
S1	y = 933.45x + 103.94	0.9995	0.359∼11.5			1.68	1.51	1.91	2.95	2.87	5.82	99.79	3.05
S2	y = 590395x + 11861	0.9997	0.0833∼3.33	53.8	179	1.25	0.43	2.58	0.779	0.750	1.54	101.14	2.73
S3	y = 5979.1x + 1339.7	0.9991	0.870∼27.8	0.832	2.08	1.45	0.68	3.46	3.68	3.70	7.35	99.32	3.01
S4	y = 7174.5x + 191.03	0.9998	0.123∼4.92	21.7	43.5	1.93	1.16	3.84	0.263	0.369	0.626	98.32	2.06
S5	y = 4331.6x + 1160.2	0.9990	0.485∼15.5	18.4	61.5	0.97	0.69	2.18	6.41	6.05	12.4	100.82	2.60
S6	y = 11038x + 3741.7	0.9990	0.515∼10.3	12.1	24.2	1.84	0.28	3.35	1.08	1.24	2.29	98.47	2.20
S7	y = 9451.4x + 4995.3	0.9991	0.907∼14.5	25.7	128.7	1.11	2.12	3.01	7.25	6.65	13.7	97.53	1.15
S8	y = 1499.9x + 316.45	0.9990	0.518∼12.4	30.2	90.7	2.41	1.57	1.88	3.88	4.14	8.01	99.70	3.13
S9	y = 4893.2x + 731.86	0.9994	0.187∼2.24	155	259	3.48	2.81	2.00	0.453	0.355	0.806	99.52	3.77
S10	y = 2133.2x + 142.91	0.9997	0.0108∼0.260	14.0	46.7	2.80	2.33	3.02	0.336	0.303	0.638	99.65	3.46
S11	y = 682.62x−1.3265	0.9991	0.0310∼1.24	1.08	2.70	4.12	4.22	4.01	0.149	0.409	0.554	98.89	3.18
S12	y = 23163x−2708.8	0.9996	0.168∼6.72	0.310	0.620	1.90	1.16	4.23	2.47	2.24	4.67	98.43	1.38