In Vivo Diuretic Activity of Hydromethanolic Extract and Solvent Fractions of the Root Bark of Clerodendrum myricoides Hochst. (Lamiaceae)

Table 1

The effect of solvent fractions of the root bark of Clerodendrum myricoides on 24 hr urine volume in rats.

Dose (mg/kg)	Urine volume (mL)
	1st hr	2nd hr	4th hr	6th hr	24th hr
AF400	0.33 ± 0.21^c2, d3, e1	0.75 ± 0.47^d3	1.83 ± 0.38^d3	2.75 ± 0.35^d3	8.33 ± 0.83^d1
AF200	0.33 ± 0.21^c2, d3, e1	1.66 ± 0.55^d1	2.33 ± 0.16^d2	3.66 ± 0.27^d2	8.50 ± 0.21^d1
AF100	1.50 ± 0.18^d1	2.33 ± 0.10	2.83 ± 0.05^d1	2.83 ± 0.52^d3	7.83 ± 0.36^d2
EAF400	1.05 ± 0.46	2.67 ± 0.42	4.33 ± 0.40	5.70 ± 0.31^e1	11.54 ± 0.50^c1,e3
EAF200	1.00 ± 0.44	2.15 ± 0.45	3.45 ± 0.31	4.45 ± 0.27	9.95 ± 0.34^e3
EAF100	1.50 ± 0.18	3.61 ± 0.35	3.74 ± 0.33	4.62 ± 0.50	9.09 ± 0.62^e1
CF400	1.58 ± 0.32	2.95 ± 0.20	3.91 ± 0.15	5.25 ± 0.21^e2	10.86 ± 0.44^b1,e3
CF200	2.25 ± 0.51	3.12 ± 0.17	2.25 ± 0.50^d2	2.50 ± 0.54^d3	10.50 ± 0.78^b1,e3
CF100	1.50 ± 0.18	2.12 ± 0.12^d1	2.91 ± 0.08^d1	3.97 ± 0.20^d1	8.12 ± 0.48^d2
F10	2.42 ± 0.32^e1	3.56 ± 0.55	4.72 ± 0.74^e2	5.9 ± 0.76^e3	11.05 ± 0.50^e3c
DW	1.38 ± 0.14	1.96 ± 0.13	2.58 ± 0.21	3 ± 0.24	7.35 ± 0.58
2%TDW	1.37 ± 0.32	2.29 ± 0.40	2.83 ± 0.30	3.32 ± 0.24	6.66 ± 0.42

Results are expressed as mean ± SEM (n = 6). Compared to AF: aqueous fraction (^a400, ^b200, ^c100 mg/kg); ^dcompared to F10: furosemide 10 mg/kg; ^ecompared to DW: distilled water; compared to EAF: ethyl acetate fraction (^a400, ^b200, ^c100 mg/kg); compared to CF: chloroform fraction (^a400, ^b200, ^c100 mg/kg); ^dcompared to F10: furosemide 10 mg/kg; ^ecompared to 2%TDW: 2% Tween 80. ¹; ²; ³.