Evidence-Based Complementary and Alternative Medicine

Research Article

In Vivo Diuretic Activity of Hydromethanolic Extract and Solvent Fractions of the Root Bark of Clerodendrum myricoides Hochst. (Lamiaceae)

Table 3

The effect of the hydromethanolic extract and solvent fractions of the root bark of Clerodendrum myricoides on urine electrolyte in rats.


Dose (mg/kg)	Urine electrolyte (mmol/l)			Saliuretic index			Na⁺/K⁺	Cl⁻/Na⁺+K⁺
Dose (mg/kg)	Na⁺	K⁺	Cl^-	Na⁺	K⁺	Cl^-	Na⁺/K⁺	Cl⁻/Na⁺+K⁺

HME400	85.33 ± 8.30	66.38 ± 14.77^b2,d2	106.83 ± 10.27^b3	0.88	0.59	0.77	1.28	0.70
HME200	121.16 ± 7.60	124.18 ± 6.34	181.83 ± 7.52^c3,d1,e1	1.26	1.11	1.31	0.97	0.74
HME100	87.66 ± 16.49	89.11 ± 7.44	114.66 ± 11.60	0.90	0.80	0.80	0.98	0.64
AF400	151.16 ± 15.09	192.50 ± 24.05^b2,c2,d1,e2	369.66 ± 36.74^b3,c3,d3,e3	1.56	1.73	2.67	0.78	1.07
AF200	116.33 ± 16.50	110.55 ± 13.85	164.33 ± 20.72	1.20	0.99	1.18	1.05	0.72
AF100	137.33 ± 15.72	102.66 ± 7.22	204.16 ± 14.85	1.42	0.92	1.47	1.33	0.85
EAF400	137.83 ± 4.84^d1,e1	114.31 ± 8.75	172.50 ± 5.61	1.36	1.01	1.27	1.20	0.68
EAF200	146.83 ± 7.66^d2,e2	110.53 ± 9.56	175.66 ± 8.72	1.45	0.98	1.29	1.33	0.68
EAF100	122.33 ± 7.51	126.45 ± 7.40	229.66 ± 27.97^d2,e1	1.21	1.12	1.69	0.96	0.92
CF400	143.33 ± 8.38^b3,e1	166.75 ± 7.74^b3,c2,e2	287.00 ± 35.24^b3,d3,e3	1.43	1.48	2.11	0.85	0.92
CF200	72.16 ± 13.77^c1	74.40 ± 12.15^d2	119.16 ± 23.22^c2	0.71	0.66	0.87	0.96	0.81
CF100	119.66 ± 6.75	107.73 ± 6.23	242.33 ± 14.16^d1,e2	1.18	0.96	1.78	1.11	1.06
F10	102.66 ± 13.13	129.58 ± 17.04	140.83 ± 11.74	1.01	1.15	1.03	0.79	0.60
DW	96.50 ± 0.67	110.88 ± 3.89	138.33 ± 3.89	—	—	—	0.87	0.67
2%TDW	100.83 ± 2.89	112.16 ± 4.33	135.66 ± 6.18	—	—	—	0.89	1.59

Results are expressed as mean ± SEM (n = 6). Compared to HME: hydromethanolic extract (^a400, ^b200, ^c100 mg/kg); compared to AF: aqueous fraction (^a400,^b200,^c100 mg/kg); ^dcompared to F10: furosemide 10 mg/kg; ^ecompared to DW: distilled water; compared to EAF: ethyl acetate fraction (^a400, ^b200, ^c100 mg/kg); compared to CF: chloroform fraction (^a400, ^b200, ^c100 mg/kg); ^dcompared to F10: furosemide 10 mg/kg; ^ecompared to 2%TDW: 2% Tween 80 in distilled water. ¹; ²; ³. Saliuretic index = Na⁺, K⁺, Cl⁻ of the test group/Na⁺, K⁺, Cl⁻ of the control group; natriuretic index = Na⁺/K⁺ and CAI index = Cl⁻/Na⁺+K⁺ in the same group.