In Vivo Diuretic Activity of Hydromethanolic Extract and Solvent Fractions of the Root Bark of Clerodendrum myricoides Hochst. (Lamiaceae)

Table 4

The effect of the hydromethanolic extract and solvent fractions of the root bark of Clerodendrum myricoides on serum electrolyte in rats.

Dose (mg/kg)	Serum electrolyte (mmol/l)
	Na^+	K^+	Cl^-
HME400	135.00 ± 4.29^c2,d1,e1	12.75 ± 3.19^b1,c2,d2	93.50 ± 2.84^c3,d2,e2
HME200	142.00 ± 1.63	5.41 ± 0.16	99.00 ± 1.29
HME100	147.66 ± 0.55	5.10 ± 0.07	103.16 ± 0.40
AF400	146.33 ± 0.98	4.46 ± 0.29^e3	102.50 ± 0.76
AF200	147.83 ± 0.87^e1	4.26 ± 0.07^e3	102.66 ± 0.71
AF100	148.00 ± 0.63^e2	3.96 ± 0.14^d1,e3	104.33 ± 0.84
EAF400	148.16 ± 0.40	4.48 ± 0.18^e2	101.00 ± 0.36
EAF200	145.00 ± 2.19	6.03 ± 0.58^c2	104.00 ± 1.23
EAF100	150.83 ± 2.18^e1	4.08 ± 0.08^e3	104.33 ± 1.22
CF400	149.83 ± 1.51^d2,e2	5.25 ± 0.47	102.83 ± 1.16
CF200	149.00 ± 0.85^d2,e1	3.98 ± 0.11^e3	100.66 ± 0.55
CF100	146.16 ± 0.47	4.03 ± 0.09^e3	101.83 ± 0.16
F10	145.16 ± 0.79	5.18 ± 0.15	101.83 ± 0.30
DW	144.00 ± 0.36	7.06 ± 0.53	101.83 ± 0.47
2%TDW	144.33 ± 0.33	6.50 ± 0.55	102.16 ± 0.47

Results are expressed as mean ± SEM (n = 6). Compared to HME: hydromethanolic extract (^a400, ^b200, ^c100 mg/kg); compared to AF: aqueous fraction (^a400, ^b200, ^c100 mg/kg); ^dcompared to F10: furosemide 10 mg/kg; ^ecompared to DW: distilled water; compared to EAF: ethyl acetate fraction (^a400 ,^b200, ^c100); compared to CF: chloroform fraction (^a400, ^b200, ^c100 mg/kg); ^dcompared to F10: furosemide 10 mg/kg; ^ecompared to 2%TDW: 2% Tween 80 in distilled water. ¹; ²; ³.