Evaluation of Methanol-Dichloromethane Extract of Stemonocoleus micranthus Harms (Fabaceae) Stem Bark for Anti-Inflammatory and Immunomodulatory Activities
Table 4
Effect of MDESm on cyclophosphamide-induced neutropenia in mice.
Treatment
Dose (mg/kg)
Total leucocyte count (cells/mm3)
Reduction in cell number after cyclophosphamide treatment (%)
Total neutrophils (cells/mm3)
Reduction in cell number after cyclophosphamide treatment (%)
Before
After
Before
After
Control (dist. water)
1 (ml/kg)
9120 ± 1563
1180 ± 162
7940 ± 1573 (87.06)
22.4 ± 3.74
24.6 ± 2.80
−2.2 ± 1.88 (−9.82)
Levamisole
50
15880 ± 3172
740 ± 163
15140 ± 3315 (95.34)
21.0 ± 3.79
14.4 ± 2.15
6.6 ± 1.80 (31.40)
MDESm
100
10575 ± 1003
3350 ± 212
7225 ± 3078 (68.32)
18.7 ± 3.42
18.7 ± 3.06
0.0 ± 5.11 (0.00)
MDESm
200
7980 ± 1285
1940 ± 821
6040 ± 1557 (75.68)
23.6 ± 3.50
21.2 ± 3.12
2.2 ± 4.03 (10.16)
MDESm
400
9925 ± 726
1675 ± 342
8250 ± 1034 (83.12)
22.2 ± 3.81
25.5 ± 2.90
−3.3 ± 5.55 (−14.86)
Mean ± SEM; n = 5, compared to control (one-way ANOVA; Dunnett’s post hoc test). MDESm = methanol-dichloromethane extract of Stemonocoleus micranthus.
