Research Article
Niao Du Kang Mixture Increases the Expression of mir-129-5p to Relieve Renal Fibrosis
Figure 3
mir-129-5p targeted PDPK1 to downregulate its expression in vitro. (a) TargetScan-predicted sequence alignment of mir-129-5p and PDPK1 3′UTRs from various species; (b) qRT-PCR results of the expression of PDPK1 after transfection; (c) Western blot results of the expression of PDPK1 after transfection; (d) the associated grey value statistics of the expression of PDPK1 after transfection; (e) Western blot results of the expression of AKT and p-AKT after transfection; (f) the associated grey value statistics of the expression of AKT after transfection; and (g) the associated grey value statistics of the expression of p-AKT after transfection. The relative expression is homogenized when statistics are performed, and the sham group is defined as 1. , , and .