Niao Du Kang Mixture Increases the Expression of mir-129-5p to Relieve Renal Fibrosis

Figure 4

Niao Du Kang mixture increased the expression of mir-129-5p in vivo. (a) qRT-PCR results of the expression of PDPK1 after treatment with TGF-β and NDK mixture-containing serum; (b) Western blot results of the expression of PDPK1 and AKT after treatment with TGF-β and NDK mixture-containing serum; (c) the associated grey value statistics of the expression of PDPK1 after treatment with TGF-β and NDK mixture-containing serum; (d) the associated grey value statistics of the expression of AKT after treatment with TGF-β and NDK mixture-containing serum; (e) the associated grey value statistics of the expression of p-AKT after treatment with TGF-β and NDK mixture-containing serum; (f) qRT-PCR results of the expression of mir-129-5p in the sham group, UUO group, positive group, NDK mixture high-dose group, NDK mixture middle-dose group, and NDK mixture low-dose group; (g) qRT-PCR results of the expression of PDPK1 mRNA in the sham group, UUO group, positive group, NDK mixture high-dose group, NDK mixture middle-dose group, and NDK mixture low-dose group; (h) Western blot results of the expression of PDPK1 and AKT in the sham group, UUO group, positive group, NDK mixture high-dose group, NDK mixture middle-dose group, and NDK mixture low-dose group; (i) the associated grey value statistics of the expression of PDPK1 in the sham group, UUO group, positive group, NDK mixture high-dose group, NDK mixture middle-dose group, and NDK mixture low-dose group; (j) the associated grey value statistics of the expression of AKT and p-AKT in the sham group, UUO group, positive group, NDK mixture high-dose group, NDK mixture middle-dose group, and NDK mixture low-dose group; and (k) NDK mixture high-dose group, NDK mixture middle-dose group, and NDK mixture low-dose group. The relative expression is homogenized when statistics are performed, and the sham group is defined as 1. , , and .

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