β-Sitosterol Protects against Myocardial Ischemia/Reperfusion Injury via Targeting PPARγ/NF-κB Signalling

Figure 1

H/R treatment suppressed cell viability, increased cell apoptosis and ROS production, and suppressed MMP in H9c2 cells. (a) Cell viability, (b) cell apoptotic rates, (c) caspase-3 activity, and (d) caspase-9 activity in control H9c2 cells or H9c2 cells subjected to H/R treatment were determined, respectively, by CCK-8, flow cytometry, and caspase-3 and -9 assays. (e) Protein levels of caspase-3, -9, and Bcl-2 in control H9c2 cells or H9c2 cells subjected to H/R treatment were measured by the western blot assay. (f) ROS production and (g) MMP of control H9c2 cells or H9c2 subjected to H/R treatment were determined by ROS production assay and MMP assay, respectively. N = 3. , , and .

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