Ethanol Extract of the Infructescence of Platycarya strobilacea Sieb. et Zucc. Induces Methuosis of Human Nasopharyngeal Carcinoma Cells

Figure 1

Effects of EPS on NPC cell viability and the cell cycle. (a) CNE1 and CNE2 cells were treated with various concentrations (0–2.0 mg/mL) of EPS for 24 h. Cell viability was detected using an MTT assay. (b) NPC cells were subjected to 1.0 mg/mL EPS for 24 h and then assessed for apoptotic cell death by flow cytometry. The total percentages of apoptotic cell death and nonapoptotic cell death were calculated, as described in the methods. (c) Cell cycle analysis of CNE1 and CNE2 cells treated with the control and 1.0 mg/mL EPS for 24 h. Representative histograms show the distribution of cells in the cell cycle for each treatment group. Data are expressed as the mean ± SD of three independent experiments, , vs the control group, as analyzed by Student’s t-test.