Ethanol Extract of the Infructescence of Platycarya strobilacea Sieb. et Zucc. Induces Methuosis of Human Nasopharyngeal Carcinoma Cells

Figure 4

The mechanisms of methuosis induction by EPS in NPC cells, as shown by network pharmacology. (a) Construction of the network map between the active ingredients of EPS and their associated targets. The network was constructed with five candidate compounds and their putative targets, which were constituents of EPS. The green nodes represent candidate compounds, and the targets are indicated by pink circles. (b) GO terms and pathways enriched in the network targets of EPS. The dot size indicates the ratio of genes in the respective pathways to the total number of tested genes, and the dot color illustrates the adjusted value of the enrichment. (c) Immunoblot analysis of senescence-related proteins in EPS-treated NPC cells. GAPDH was used as a control for these proteins. (d) The GO terms in the biological process categories at all levels enriched in downstream target genes of c-FOS. (e) Construction of the GO BP terms. The histogram was generated by connecting the candidate GO terms and their targeted genes, which were related to methuosis.

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