Erchen Plus Huiyanzhuyu Decoction Inhibits the Growth of Laryngeal Carcinoma in a Mouse Model of Phlegm-Coagulation-Blood-Stasis Syndrome via the STAT3/Cyclin D1 Pathway

Figure 5

Effects of EHD on the STAT3/cyclin D1 pathway in the tumour tissues of nude mice with LC and PCBS syndrome. Western blot analysis of the levels of STAT3, p-STAT3, cyclin D1, and P27 in nude mice treated with 14.44, 28.87, or 57.74 g/kg EHD and 2 mg/kg DDP for 3 weeks. GAPDH was used to ensure equal loading of proteins in each lane. All data are presented as the mean ± SD of three independent experiments. (a) Treatment with 28.87 and 57.74 g/kg EHD and 2 mg/kg DDP inhibited STAT3 protein expression. (b) Treatment with 28.87 and 57.74 g/kg EHD and 2 mg/kg DDP also decreased p-STAT3 protein expression. (c) Different concentrations of EHD and 2 mg/kg DDP downregulated cyclin D1 protein expression. (d) EHD upregulated the P27 protein levels in a dose-dependent manner, but 2 mg/kg DDP had no effect on the P27 protein levels. , , and vs. the control group (0 g/kg EHD).