Activation of the Nrf2/HO-1 Pathway by Amomum villosum Extract Suppresses LPS-Induced Oxidative Stress In Vitro and Ex Vivo

Figure 6

Effects of AVEE on proinflammatory gene expression in murine peritoneal macrophages. Murine macrophages were incubated with various concentrations of AVEE (25, 50, 75, 100, 150, and 200 μg/mL) for 24 h. Cell viabilities were measured using an MTT assay (a). Isolated peritoneal murine macrophages were incubated with AVEE (100 μg/mL) for 3 h and stimulated with LPS (1 μg/mL). IL-1β (b), IL-6 (c), COX-2 (d), and TNF-α (e) mRNA expressions are presented as fold increases versus treatment naïve controls. Results are presented as the means ± SDs of three independent experiments. versus treatment naïve controls. versus LPS treated controls.

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