Establishment of Fluorescence Sensitization Method for Hydroxysafflor Yellow A

Figure 3

(a, d) Absorption spectra and (b, e) fluorescence spectra of HSYA in the presence of common cations and anions. (c, f) The maximum fluorescence intensity in the presence of various cations and anions. The cations included: Na⁺, K⁺, Mg²⁺, Ca²⁺, Fe²⁺, Fe³⁺, Cu²⁺, Zn²⁺, Co²⁺, Ni²⁺, Mn²⁺, NH₄⁺, Sn²⁺, Sr⁺, Cr³⁺, and Ba²⁺. The anions included: F⁻, Cl⁻, Br⁻, I⁻, , CH₃COO⁻, EDTA, , , , , , , , , CH₄N₂O, and . All spectra were measured in PBS ( = 10 mM, pH = 7) solution at room temperature, with excitation at 450 nm.

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