Establishment of Fluorescence Sensitization Method for Hydroxysafflor Yellow A

Figure 7

(a) Fluorescence spectra of HSYA at various concentrations (0∼1000 μM) in the borax-HSYA sensitization system containing 4 mM borax. (b) The fluorescence intensity at 554 nm varied with HSYA concentrations ranging from 0 to 10 μM (F₀ and F are the fluorescence intensities before and after adding HSYA into the sensitization system). (c) Linear relationship between the increase in fluorescence intensity at 554 nm and HSYA concentration (0∼10 μM). All spectra were measured in PBS ( = 10 mM, pH = 7) solution at room temperature, with excitation at 450 nm.

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