Glycyrrhetinic Acid-Induced MiR-663a Alleviates Hepatic Stellate Cell Activation by Attenuating the TGF-β/Smad Signaling Pathway

Figure 1

miR-663a was downregulated in TGF-β1-treated HSCs, and miR-663a inhibited HSC proliferation. (a) miR-663a expression was lower in LX2 cells than in L02 cells (). (b) and (c) MiR-663a expression (b) was inhibited, and α-SMA and COL1A2 protein expression levels (c) were induced by 24 h and 48 h of TGF-β1 (10 ng/ml) stimulation in LX2 cells. The miRNA level of miR-663a and protein levels of α-SMA and COL1A2 were examined by RT-qPCR and western blot. , compared with LX2 cells. (d) Inhibitory effects of miR-663a overexpression on HSC proliferation. LX2 cells were transfected with miR-663a mimics or miRNA mimic negative control (miR-NC) for 24 h, 48 h, 72 h, and 96 h using lipofectamine 3000. Cell proliferation is detected by MTS. Each value is the mean ± SD of three experiments. , compared with the control.

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