Glycyrrhetinic Acid-Induced MiR-663a Alleviates Hepatic Stellate Cell Activation by Attenuating the TGF-β/Smad Signaling Pathway

Figure 3

Overexpression of miR-663a inhibited the TGF-β/Smad signaling pathway in HSCs. (a) and (b) LX2 cells were transfected with miR-663a mimics or miRNA mimic negative control (miR-NC) for 24 h and treated with TGF-β1 (10 ng/ml) for 48 h. The protein expression levels of TGF-β1, TGF-βRI, Smad2, Smad3, Smad4, COL1A2, and α-SMA and the protein levels of p-Smad2 and p-Smad3 in the cytoplasm and nucleus were measured by western blot. Each value is the mean ± SD of three experiments. , compared with the control group.

(a)

(b)