Glycyrrhetinic Acid-Induced MiR-663a Alleviates Hepatic Stellate Cell Activation by Attenuating the TGF-β/Smad Signaling Pathway

Figure 6

Inhibition of miR-663a overcame the GA-mediated suppression of the TGF-β/Smad signaling pathway. (a) and (b) The protein expression levels of TGF-β1, TGF-βRI, Smad2, p-Smad2, Smad3, p-Smad3, and Smad4 and the protein levels of COL1A2 and α-SMA in the cytoplasm and nucleus were detected by western blot. LX2 cells were transfected with miR-663a inhibitors (anti-miR-663a) or negative control inhibitors (anti-miR-NC) for 24 h and then treated with GA and TGF-β1 for 48 h. GAPDH was used as an internal control. Each value is the mean ± SD of three experiments. , compared with the control group.

(a)

(b)