Preliminary Antimicrobial Profile of Solanum incanum L.: A Common Medicinal Plant

Table 1

Phytochemical tests.

Tests	Procedures
Alkaloids	About half (0.5) gram of each crude extract was stirred in 5 mL of 1% aqueous HCl on a steam bath, cooled and filtered. Then, 1 mL of the filtrate was treated with a few drops of Wagner’s reagent to observe the formation of yellow or brown precipitate
Flavonoids	Five to ten (5 to 10) drops of dil. HCl and small piece Mg powder was added to 0.5 mL of each extract and boiled for few minutes to observe reddish pink or brown color formation
Glycosides	Aqueous NaOH solution was added to each extract and was dissolved in 1 mL of water to observe the formation of yellow color
Saponins	About half (0.5) gram of each extract was mixed, shaken with distilled water in test tube to see the formation and persistency of froth for 15 minutes
Steroids	About 0.5 mL of each extract was dissolved in 5 mL of chloroform. Then, 5 mL conc. H2SO4 was added by sides of the test tube to observe turning of the upper layer to red color and H2SO4 layer to yellow with green fluorescence
Terpenoids	Five (5) mL of each extract was mixed in 2 mL of chloroform. Then, a layer was formed by adding 3 mL of conc. H2SO4 to observe reddish color formation in the interface