Research Article
Traditional Chinese Medicine Prescriptions Decrease Diarrhea Rate by Relieving Colonic Inflammation and Ameliorating Caecum Microbiota in Piglets
Table 2
Primers used in this study.
| | Target | GenBank number | Primers sequence (5′-3′) |
| | GAPDH | NM_001206359 | F: ACTCACTCTTCCACTTTTGATGCT | | R: TGTTGCTGTAGCCAAATTCA | | TLR4 | NM_001113039.1 | F: GCCATCGCTGCTAACATCATC | | R: CTCATACTCAAAGATACACCATCGG | | MyD88 | NM_001099923.1 | F: TGGTAGTGGTTGTCTCTGATGA | | R: TGGAGAGAGGCTGAGTGCAA | | NF-κB | NM_001048232.1 | F: CTCGCACAAGGAGACATGAA | | R: ACTCAGCCGGAAGGCATTAT | | IL-6 | NM_001252429.1 | F: TGGCTACTGCCTTCCCTACC | | R: CAGAGATTTTGCCGAGGATG | | IL-8 | NM_213867.1 | F: TTCGATGCCAGTGCATAAATA | | R: CTGTACAACCTTCTGCACCCA | | IL-10 | HQ026020.1 | F: GCTGAAGACCCTCAGGCTGA | | R: GCTGAAGACCCTCAGGCTGA | | TNF-α | NM_214022.1 | F: CCAATGGGCAGAHTGGGTATG | | R: TGAAGAGGACCTGGGAGTAG |
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Note. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; TLR4, toll-like receptors 4; MyD88, myeloid differentiation factor 88; NF-κB, nuclear factor-κB; IL-6, interleukin-6; IL-8, interleukin-8; IL-10, interleukin-10; TNF-α, tumor necrosis factor-α.
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