Kai-Xin-San, a Chinese Herbal Decoction, Accelerates the Degradation of β-Amyloid by Enhancing the Expression of Neprilysin in Rats

Figure 1

Two experimental protocols. (a) was developed for the MWM test and HE staining. At day 14, Aβ was injected into rat hippocampus in the Aβ group as well as the KXS + Aβ group, whereas 0.9% saline was injected in the sham group. After the MWM test from day 15 to day 20, HE was developed on day 21. (b) was developed for Aβ measurement and western blot for NEP, ACE, and ECE. ELISA for Aβ measurement was developed from day 14.5 to day 21 (12 h, 24 h, 48 h, 96 h, and 168 h after Aβ injection). Rat brains were removed for western blotting on day 21.

(a)

(b)