Antimicrobial, Cytotoxic, and Antioxidant Potential of a Novel Flavone “6,7,4′-Trimethyl Flavone” Isolated from Wulfenia amherstiana

Table 9

Brine shrimp lethality assay (cytotoxic activity of TMF).

Material	Dose (μg/ml/ml)	No. of shrimps	No. of survivors	Mortality %	LD50 (μg/ml)
6,7,4′-trimethyl flavone	1000	30	3	90	127.014
	750	30	6	80
	500	30	9	70
	100	30	15	50
	50	30	21	30
Positive control etoposide	1000	30	6	80	8.997
	750	30	8	76.7
	500	30	8	73.3
	100	30	10	66.7
	50	30	12	60
Negative control sea water	1000	30	30	0	—
	750	30	30	0
	500	30	30	0
	100	30	30	0
	50	30	30	0