Research Article

Abdominal Massage Reduces Visceral Hypersensitivity via Regulating GDNF and PI3K/AKT Signal Pathway in a Rat Model of Irritable Bowel Syndrome

Figure 2

Effects of abdominal massage on the expression of CHAT, nNOS, and PGP 9.5 (immunofluorescence staining). Orange is positive protein, and DAPI (blue) is used to stain the cell nucleus (×400). The rats are divided into the control group, the model group, and the abdominal massage (AM) group.