Comparative Assessment of the Antioxidant Activities among the Extracts of Different Parts of Clausena lansium (Lour.) Skeels in Human Gingival Fibroblast Cells

Figure 4

ROS accumulation was measured by DCFH-DA fluorescent dye. Cells were loaded with 20 µM DCFH-DA for 20 min and then washed with PBS and re-incubated with 1 mM H₂O₂ and 500 µg/ml of the different wampee extracts. Fluorescence was then measured at regular intervals for 6 h Three independent experiments were performed to obtain the mean fluorescence values and standard deviations. indicates a significant difference between the H₂O₂-treated and wampee extract-co-treated cells ().

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