ErMiao San Inhibits Angiogenesis in Rheumatoid Arthritis by Suppressing JAK/STAT Signaling Pathways

Figure 3

EMS suppresses VEGF₁₆₅-induced vessel sprout formation ex vivo. Rat aortic rings were placed on a Matrigel drop in 48-well plates for 9 days and covered with an additional drop of Matrigel. Serum-free H-DMEM medium (400 μl) with various concentrations of EMS (0.2, 0.4, and 0.8 mg/mL) was added to each well. VEGF (20 ng/mL) was used as a positive control. Photographs of vessel sprout formation were obtained under an inverted microscope (magnification ×100) (a). The number of microvessel vessels (b), microvessel length (c), and microvessel area (d) was counted, respectively. Three independent experiments were performed. Data are represented as means ± SEM (n = 3). , compared to the control group. , , and , compared to the VEGF-treated group.

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(b)

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