ErMiao San Inhibits Angiogenesis in Rheumatoid Arthritis by Suppressing JAK/STAT Signaling Pathways

Figure 5

EMS reduces the expression levels of various proangiogenic activators in collagen-induced arthritis (CIA) mice and in HUVECs. CIA mice were orally administered EMS (5 g/kg) for 20 days from the day of second immunization. The levels of IL-1β (a), IL-6 (b), and TNF-α (c) in sera of mice were measured by enzyme-linked immunosorbent assay (ELISA). The expression of phosphorylation of JAK1 (d), STAT1 (e), and STAT6 (f) in joint synovia of CIA mice was detected by Western blot. HUVECs were starved in 10% FBS medium for 48 h pretreated with EMS for 2 h and then stimulated with VEGF (20 ng/mL) for 15 min before collected. The levels of phosphorylation of JAK1 (g), STAT1 (h), and STAT6 (i) were analyzed by Western blot, too. All experiments were done in triplicate. Mean ± SEM (n = 3) was calculated from independent experiments. , , compared to the control group; , , and , compared to the CIA/VEGF-induced group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)