Research Article

Radix Angelica Sinensis and Radix Hedysari Ultrafiltration Extract Protects against X-Irradiation-Induced Cardiac Fibrosis in Rats

Figure 5

RAS-RH induces apoptosis in primary rat cardiac fibroblasts after X-irradiation. (a) Flow cytometry analysis of primary rat cardiac fibroblast apoptosis in the Con, X-ray, and RAS-RH + X-ray groups. (b) The bar graph shows a statistical analysis of image (a) results. (c) Western blotting images for TNF-α and caspase-3 protein expression in the Con, X-ray, and RAS-RH + X-ray groups. (d) Relative TNF-α and caspase-3 protein expression levels were analyzed by Image-Pro Plus 6.0. (e) Quantitative real-time RT-PCR analysis of TNF-α and caspase-3 mRNA levels (n = 3, vs. the control group; , vs. the X-ray group).
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