Enriched-Baicalein Attenuates Allergy in Cells and Mice

Figure 1

Cytotoxicity of baicalein against RBL-2H3 mast cells (a) and inhibitory rate on the release of β-hexosaminidase by baicalein treatment in RBL-2H3 mast cells. (b) Cytotoxicity of SB extract and baicalein (50, 100, 200, and 500 μg·mL⁻¹, respectively) was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on RBL-2H3 mast cells after 24 h. The cytotoxicity was expressed as a percentage. The inhibitory rates on the release of β-hexosaminidase by baicalein treatment were conducted in IgE-stimulated RBL-2H3 mast cells. After seeding RBL-2H3 cells (plate (2 × 10⁵ cells·well⁻¹)) for 24 hrs, anti-DNP (dinitrophenol) mouse IgE (0.5 μg·mL⁻¹) was supplemented. After 24 h, Tyrode’s buffer (100 μL) was added, the plate was incubated for 30 min, and Tyrode’s buffer (100 μL) containing 50 μg/mL DNP-bovine serum albumin (BSA) was replaced. After incubation of 1 h, the 50 μL of the supernatant was collected and mixed with substrate solution (p-nitrophenyl-N-acetyl-β-glucosaminide, 2 mM) of 50 μL. After 3 hrs, a stop solution of 100 μL was added, and the absorbance of the plate was read at 405 nm. Dimethyl sulfoxide (0.01%) was used for all no-drug control samples. All measurements were made in triplicate and all values are represented as mean ± SD. SB, Scutellaria baicalensis Georgi extract. , , and .

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