Research Article
Protective Effects of Grape Seed Proanthocyanidins on the Kidneys of Diabetic Rats through the Nrf2 Signalling Pathway
Table 1
Primer sequences for quantitative real-time polymerase chain reaction.
| | Name | Primer | Sequence | Size (bp) |
| | GAPDH | Forward | 5ʹ-ACAGCAACAGGGTGGTGGAC-3ʹ | 253 | | Reverse | 5ʹ-TTTGAGGGTGCAGCGAACTT-3ʹ |
| | HO-1 | Forward | 5ʹ-GCATGTCCCAGGATTTGTCC-3ʹ | 192 | | Reverse | 5ʹ-GGTTCTGCTTGTTTCGCTCT-3ʹ |
| | GST | Forward | 5ʹ-ACCTTTTGAGACCCTGCTGT-3ʹ | 224 | | Reverse | 5ʹ-GACGGTTCAAATGGTCAGGG-3ʹ |
| | Nrf2 | Forward | 5ʹ-CCCATTGAGGGCTGTGAT-3ʹ | 247 | | Reverse | 5ʹ-TTGGCTGTGCTTTAGGTC -3ʹ |
| | NQO1 | Forward | 5ʹ-GATGGGAGGTGGTCGAATCT-3ʹ | 197 | | Reverse | 5ʹ-TATCACCAGGTCTGCAGCTT-3ʹ |
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GST: glutathione S-transferase; Nrf2: nuclear factor erythroid 2-related factor 2; NQO1, NAD (P)H quinone oxidoreductase 1.
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