Study of Jianpi Mixture on Intestinal Microbiota of Diarrhea Irritable Bowel Syndrome Mice

Figure 2

Electrophoretogram of PCR amplification of bacterial DNA in 60 samples. (a) Electrophoretogram of secondary PCR amplification of fecal bacterial DNA in 20 samples before treatment. Each band of 1∼20 represents the molecular weight of fecal bacterial DNA in the sample of mice before treatment. Marker is DL2000. The top to bottom band is 2000 bp, 1000 bp, 750 bp, 500 bp, 250 bp, and 100 bp; the loading is 3 µL, and the bright band is 30 ng/µL; the remaining strips are all 10 ng/µL. (b) Electrophoretogram of secondary PCR amplification of bacterial DNA in 40 samples after treatment. Each band of 1∼20 represents the molecular weight of bacterial DNA in the fecal sample of the mice after treatment, each band of 21–40 represents the molecular weight of bacterial DNA of the colonic mucosa of the mice after treatment, and marker is DL2000. The top to bottom band is 2000 bp, 1000 bp, 750 bp, 500 bp, 250 bp, and 100 bp; the loading is 3 µL, the bright band is 30 ng/µL, and the remaining bands are 10 ng/µL.

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