Research Article

Antiaging Activity of Peptide Identified from Fermented Trapa Japonica Fruit Extract in Human Dermal Fibroblasts

Figure 4

Effects of TJE, FTJ, and PEP on TNF-α-induced MMP-1 and MMP-9 mRNA levels in HDFs. Cells were pretreated with TJE, FTJ, and PEP at a concentration of 1 μg/mL for 1 h and then stimulated with 20 ng/mL TNF-α for 24 h. The mRNA expression levels of MMP-1 and MMP-9 were measured using RT-PCR. GAPDH was used as the internal control. ; vs. TNF-α-treated group.