Anti-Inflammatory Effects of Modified Buyang Huanwu Decoction

Figure 6

Both mBHD-1 and mBHD-2 inhibit NO secretion by regulating the p38 signaling pathway in LPS-exposed RAW264.7 cells. Cells were pretreated with SP600125 (a JNK inhibitor) or SB203580 (a p38 inhibitor) with or without BHD, mBHD-1, or mBHD-2 for 1 h and then were stimulated with LPS (100 ng/mL). After 24 h, NO was detected in conditioned media using a NO detection kit (a), and expression of iNOS and Cox-2 was measured in total protein using western blotting (b). LPS + SP: LPS and SP600125 treatment; LPS + SB: LPS and SB203580 treatment. ; vs. LPS; vs. LPS + SP. Statistical differences were analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. Error bars indicate SEM.

(a)

(b)