Research Article
Antimalarial Activities of Hydromethanolic Crude Extract and Chloroform Fraction of Gardenia ternifolia Leaves in Plasmodium berghei Infected Mice
Table 1
Phytoconstituents of hydromethanolic crude extract of G. ternifolia leaves.
| Phytochemical | Type of test | Appearance when positive | Result |
| Alkaloids | Wagner’s and Mayer’s tests | Reddish brown and white creamy ppt | + | Anthraquinones | (Borntrager’s test) chloroform + NaOH | Red colour | + | cardiac glycosides | (Keller–Killani test) glacial acetic acid + ferric chloride + concentrated sulfuric acid | A brown ring | - | Flavonoids | Alkaline reagent test | Colourless | + | Phenols | Ferric chloride | Bluish black | + | Resins | Ethanol + distilled water | Formation of a precipitate | - | Saponins | Foam test | Foam | + | Sterols | (Liebermann–Burchard test) chloroform + acetic acid + sulphuric acid | Blue green ring | + | Tannins | Ferric chloride | A brownish green or blue black coloration | + | Terpenoids | (Salkowski test) sulphuric acid + chloroform | Reddish brown | + |
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(+) indicates presence; (−) indicates absence; ppt.: precipitate.
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