Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats

Table 1

Three categories of behaviours of Sprague Dawley rats were observed on days 0, 3, 7, 11, and 14 of after wounding. Three criteria listed are withdrawal pain response (wound licking/scratching), sleeping/resting, and cage exploration (walking/climbing). All rats showed similar pattern of behaviour throughout healing process. Seven treatment groups were tested which were 0.1%, 0.5%, and 1.0% of polychaete ointment (PO), gamat (15.0%), acriflavine (0.1%), cetomacrogol emulsifying ointment (negative control), and untreated group.

Treatment	Day/Activity		0	3	7	11	14
Untreated	Wound licking/scratching		+
	Sleeping/resting			+	+
	Cage exploration	Walking			+	+	+
		Climbing				+	+
0.1% PO	Wound licking/scratching		+
	Sleeping/resting			+	+
	Cage exploration	Walking			+	+	+
		Climbing				+	+
0.5% PO	Wound licking/scratching		+
	Sleeping/resting			+	+
	Cage exploration	Walking			+	+	+
		Climbing				+	+
1.0% PO	Wound licking/scratching		+
	Sleeping/resting			+	+
	Cage exploration	Walking			+	+	+
		Climbing				+	+
Negative control	Wound licking/scratching		+
	Sleeping/resting			+	+
	Cage exploration	Walking			+	+	+
		Climbing				+	+
Gamat (15.0%)	Wound licking/scratching		+
	Sleeping/resting			+	+
	Cage exploration	Walking			+	+	+
		Climbing				+	+
Acriflavine (0.1%)	Wound licking/scratching		+
	Sleeping/resting			+	+
	Cage exploration	Walking			+	+	+
		Climbing				+	+

+ indicates presence of behaviour activity.