Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats

Table 6

Tentative metabolites identified in D. claparedii aqueous extract using ¹H NMR.

Metabolite	Chemical shift ( ppm)
Amino acids
Betaine	3.16 (s), 3.76 (s)
Glycine	3.55 (s)
Histidine	3.08 (m), 3.29 (m), 3.98 (m), 7.11 (s), 7.19 (br s)
Methionine	2.02 (m), 2.08 (s), 2.17 (m), 2.66 (t, J = 8.0 Hz), 3.89 (m)
Taurine	2.83 (t, J = 6.4 Hz), 3.10 (t, J = 6.4 Hz)
Tyrosine	3.03 (m), 3.19 (m), 3.94 (m), 6.86 (m), 7.19 (m)
Halogenated aromatics
2-Bromophenol	6.98 (d, J = 6.8 Hz), 7.86 (d, J = 7.2 Hz)
4-Bromophenol	6.79 (d, J = 8.4 Hz), 7.10 (d, J = 8.4 Hz)
2,4,6-tribromophenol	7.77 (s)
Organic acids
3-Hydroxyisovalerate	1.27 (s), 2.32 (s)
3-Hydroxybutyrate	1.17 (d, J = 6.4 Hz), 2.15 (m), 2.27 (m), 4.14 (m)
Acetate	1.87 (s)
Lactate	1.33 (d, J = 8 Hz), 4.13 (m)
Vitamin
Trigonelline	4.41 (s), 8.09 (m), 8.96 (m), 9.22 (m)
Others
Choline	3.16 (s), 3.48 (m), 3.92 (m)
Creatinine	3.06 (s), 4.05 (s)
Guanidinoacetate	3.76 (s)
Hypoxanthine	8.04 (s), 8.14 (s)
Trimethylamine	2.97 (s)
Trimethylamine N-Oxide	3.28 (s)

Note. The type of peaks is listed for each metabolite, where s = singlet, br s = broad singlet, d = doublet, t = triplet and m = multiplet. The coupling constant, J, is a measure of the interaction between a pair of protons.