Preventive Effects of <i>Chrysanthemum coronarium L.</i> Extract on Bone Metabolism <i>In Vitro</i> and <i>In Vivo</i>

<div>The effect of <i>Chrysanthemum coronarium</i> on receptor activator nuclear-factor <i>κ</i>B ligand-induced osteoclastic gene expression in the murine macrophage-like cell line RAW 264.7. (A–D) The cells were cultured with receptor activator nuclear-factor <i>κ</i>B ligand (RANKL) (50 ng/mL) at various concentrations of CC for 4 d and osteoclast-specific gene expression was determined. (a) Cathepsin K. (b) Tartrate-resistant acid phosphatase (TRAP), (c) dendritic cell-specific transmembrane protein (Dc-Stamp), and (d) calcitonin receptor (CTR) expressions were analyzed using RT-qPCR. RNA expression levels were normalized to those of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). 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403 369 403C428 403 482 380 482 289Z"></path></g><g transform="matrix(.013,0,0,-0.013,16.288,0)"><path d="M512 -3V55L134 254V256L512 456V514L75 281V230L512 -3Z"></path></g><g transform="matrix(.013,0,0,-0.013,27.551,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,33.791,0)"><path d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z"></path></g><g transform="matrix(.013,0,0,-0.013,36.755,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,42.995,0)"><path d="M153 550H386L412 615L406 623H120L82 318C104 327 142 338 184 338C294 338 347 275 347 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Evidence-Based Complementary and Alternative Medicine

fig5

Figure 5

Figure 5: Preventive Effects of <i>Chrysanthemum coronarium L.</i> Extract on Bone Metabolism <i>In Vitro</i> and <i>In Vivo</i> 

Evidence-Based Complementary and Alternative Medicine

Preventive Effects of Chrysanthemum coronarium L. Extract on Bone Metabolism In Vitro and In Vivo

Figure 5