Research Article
Preventive Effects of Chrysanthemum coronarium L. Extract on Bone Metabolism In Vitro and In Vivo
Table 1
Sequences of target gene-specific primers used in RT-qPCR.
| | Target gene | Sequence |
| | Cathepsin K | Sense
Antisense | 5′-CAG TAG CCA CGC TTC CTA TCC -3′
5′-ACT GGG TGT CCA GCA TTT CC-3′ |
| | ctr | Sense
Antisense | 5′-GGAAAAGAAGTGCCCGCTGA-3′
5′-TCTCCACTGA AAGCCAGCAG-3′ |
| | trap | Sense
Antisense | 5′- GCA GCC AAG GAG GAC TAC-3′
5′-CCC ACT CAG CAC ATA GCC-3′ |
| | DC-stamp | Sense
Antisense | 5′- TAT CTG CTG TAT CGG CTC A-3′
5′-AGA ATA ATA CTG AGA GGA ACC CA-3′ |
| | alp | Sense
Antisense | 5′- CGGGACTGGTACTCGGATAA -3′
5′-TGAGATCCAGGCCATCTAGC-3′ |
| | runx2 | Sense
Antisense | 5′- CGGCCCTCCCTGAACTCT -3′
5′-TGCCTGCCTGGG ATCTGTA-3′ |
| | opg | Sense
Antisense | 5′-GTTCTTGCACAGCTTCACCA-3′
5′-AAACAGCCCAGTGACCATTC-3′ |
| | rankl | Sense
Antisense | 5′-CCCTGAAAGGCTTGTTTC-3′
5′-CCATGAAAACGCAGATTTG-3′ |
| | gapdh | Sense
Antisense | 5′-AAA TGG TGA AGC TCG CTC TG-3′
5′-TGA AGG GGT CGT TGA TGG-3′ |
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ctr, calcitonin receptor; trap, tartrate-resistant acid phosphatase; DC-stamp, dendritic cell-specific transmembrane protein; alp, alkaline phosphatase; runx-2, runt-related transcription factor-2; opg, osteoprotegerin; rankl, receptor activator nuclear factor-κB ligand; gapdh, glyceraldehyde 3-phosphate dehydrogenase.
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