Effect of Ephedra Herb on Erlotinib Resistance in c-Met-Overexpressing Non-Small-Cell Lung Cancer Cell Line, H1993, through Promotion of Endocytosis and Degradation of c-Met

Figure 4

The effect of EHE on overexpression of c-Met and EGFR in H1993 cells. The expression levels of c-Met, pMet, EGFR, and pEGFR in H1993 cells at 4 h after treatment of 25, 50, 100, and 200 µg/ml EHE were analyzed by western blotting (a). Cells were incubated for 0, 0.25, 0.5, 2, 4, 8, and 24 h in RPMI-1640 medium containing 100 µg/ml EHE. The expression levels of c-Met, pMet, EGFR, and pEGFR were then analyzed by western blotting (b).

(a)

(b)