Effect of Ephedra Herb on Erlotinib Resistance in c-Met-Overexpressing Non-Small-Cell Lung Cancer Cell Line, H1993, through Promotion of Endocytosis and Degradation of c-Met

Figure 5

Expression of c-Met and pMet in H1993 cells after treatment of Pitstop-2 and EHE. H1993 cells were incubated for 5 min at 37°C in RPMI-1640 or RPMI-1640 containing 10 µM or 20 µM Pitstop-2. Subsequently, 50 µg/ml EHE was added, and the cells were incubated for 15 min. The expression levels of c-Met, pMet, and GAPDH were analyzed by western blotting (a). The density of each band was analyzed with an ImageQuant Las 4000 mini system without saturating the signal. The value is expressed as follows: ((the density of the c-Met or pMet band from cells treated with the test drug/the density of the GAPDH band in cells treated with the test drug)/(the density of the c-Met or pMet band in control cells/the density of the GAPDH band in the control cells)) ((b) and (c)). Each error bar represents the average (n = 3) ± S.D. Data were analyzed by 1-way ANOVA (B : DF = 2, F(2, 9) = 29.26, and ; C : DF = 2, F(2, 6) = 9.495, and ). Significant differences were determined by Dunnett’s test; B: vs. control, C: vs. 50 µg/ml EHE.

(a)

(b)

(c)